Accession Number:

ADA539442

Title:

Investigating The Anti-apoptotic Effects of Shigella Flexneri Infection In Epithelial Cells

Descriptive Note:

Doctoral thesis

Corporate Author:

UNIFORMED SERVICES UNIV OF THE HEALTH SCIENCES BETHESDA MD F EDWARD HEBERT SCHOOL OF MEDICINE

Personal Author(s):

Report Date:

2009-08-13

Pagination or Media Count:

309.0

Abstract:

Shigella flexneri is a Gram-negative, facultative intracellular pathogen that causes bacillary dysentery by invading the colonic epithelium. Previous research has shown that Shigella induces a rapid cell death in macrophages. In infected epithelial cells however only a stress response is observed and the eukaryotic cells remain viable during infection. Since S. flexneri utilizes epithelial cells for replication and survival, the hypothesis of this dissertation is that S. flexneri inhibits apoptosis in epithelial cells in order to establish a replicative niche inside the host. An apoptosis assay was developed by adding staurosporine, a chemical inducer of the intrinsic pathway of apoptosis, to the standard invasion assay.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE