Accession Number:

ADA533810

Title:

Final Report for contract Number W911NF-06-1-0515 (University of Florida)

Descriptive Note:

Final rept. 1 Oct 2006-30 Sep 2009

Corporate Author:

FLORIDA UNIV GAINESVILLE

Personal Author(s):

Report Date:

2010-07-15

Pagination or Media Count:

7.0

Abstract:

We have been investigating an electrochemical single-molecule counting experiment called nanopore resistive-pulse sensing. The sensor element is a conically shaped gold nanotube embedded in a thin polymeric membrane. We have been especially interested in counting protein molecules using these nanotube sensors. This is accomplished by placing the nanotube membrane between two electrolyte solutions, applying a transmembrane potential difference, and measuring the resulting ionic current flowing through the nanopore. In simplest terms, when a protein molecule enters and translocates the nanopore, it transiently blocks the ion current, resulting in a downward current pulse. In this way, single-molecule pore-translocation events are counted as individual current pulses. The frequency of theses current-pulse events is proportional to the concentration of the analyte, and the identity of the analyte is encoded in the current-pulse signature, as defined by the average magnitude and the duration of the current pulses. See attachment.

Subject Categories:

  • Physical Chemistry
  • Refractory Fibers

Distribution Statement:

APPROVED FOR PUBLIC RELEASE