Accession Number:

ADA533680

Title:

Defining Genomic Changes in Triple Negative Breast Cancer in Women of African Descent

Descriptive Note:

Annual rept. 1 Jun 2009-31 May 2010

Corporate Author:

MIAMI UNIV FL

Personal Author(s):

Report Date:

2010-07-01

Pagination or Media Count:

15.0

Abstract:

Breast cancer BC is the second leading cause of cancer death among African-American AA women, with mortality 20 greater than that in Caucasians Cauc. However, the basis for such disparity remains an enigma. Recent observations from our laboratory suggest the involvement of unidentified genes contributing to AA BC risk. Matched tumor and normal FFPE samples from Cauc and AA patients were obtained from the UM Sylvester Breast Tissue Bank UMS BTB under an IRB-approved protocol. Based on analysis of 22,000 transcripts, ethnic specific gene expression patterns were identified that may provide important new insights into molecular mechanisms of ethnic subtype differences in clinical outcomes. We propose to extend these preliminary findings to a large African tumor bank available via collaboration between Drs. Peter A. Bird Kijabe, Kenya and Mark Pegram UM Sylvester. Additionally, we propose to analyze chromosomal alterations associated with gene expression differences utilizing array cGH in collaboration with Alan Ashworth, England. This work will contribute to development of rationale designs of preventive, predictive and therapeutic measures for BC in different ethnicities, and thus, a significant reduction in current ethnicspecific disparities in BC incidence, morbidity and mortality. Hypothesis Discrete genomic alterations and gene expression changes will be identified and shared between triple negative tumor specimens within an ethnic group, i.e., North AmericansAfrican decent and Kenya. Aim I Analyze and compare genome-wide differences in gene expression in BC samples of AA ancestry vs. native African Kijabe samples Drs. Pegram, Baumbach, Bird, Halsey. Aim II Investigate possible chromosomal alterations associated with gene expression differences Drs. Pegram, Baumbach, Ashworth. Aim III Analyze ancestry of each sample using a panel of ancestry-informative DNA markers Drs. Kittles, Baumbach. Synergy Statement.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE