Accession Number:

ADA533672

Title:

Human Neural Cell-Based Biosensor

Descriptive Note:

Quarterly technical rept. 11 Oct-11 Dec 2010

Corporate Author:

ARUNA BIOMEDICAL INC ATHENS GA

Report Date:

2010-12-10

Pagination or Media Count:

5.0

Abstract:

Human neural progenitor cells have a strong potential for use as cell-based biosensors for environmental toxins. The overall goal of this project is to develop a human neural cell based biosensor using ArunAs neural progenitor cell line, hNP1trademark. In this report, we detail the progress in development for the following areas 1 methods to isolate and enrich for neural progenitors using cell surface markers, 2 methods to directed differentiation of progenitors into dopaminergic neurons, motoneurons and astrocytes using defined medium conditions, 3 development of cell-based methods to detect botulinum toxin, and 4 development of fluorescence based assays for proliferation, mitochondrial function and reactive oxygen species generation as sensor elements.

Subject Categories:

  • Medicine and Medical Research
  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE