Dual Delivery of Growth Factors and or Antibiotics from Chitosan-Composites for Bone Regeneration
Final rept. 1 Aug 2009-30 Sep 2010
MEMPHIS UNIV TN
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This one year project investigated in vitro the ability of microsphere-based chitosan-calcium sulfate composites to locally deliver growth factor and antibiotics to heal bone and prevent infection in traumatic musculoskeletal injuries. Antibiotic, vancomycin vanc was loaded into calcium sulfate CaS and microspheres loaded with either vanc or bone morphogenetic protein 2 BMP-2. In vitro release was evaluated over 6 weeks and biological activity and interactions of released factors were evaluated in cultures. Composites released vanc at levels 40microgml that killed S. aureus for 18 days. BMP-2 release from microspheres was reduced to 1ngmlday when combined with CaS as compared to plain microspheres 9ngmlday for days 6-10 elution. This may be due to high affinity of BMP for calcium and hence may serve to retain BMP in wound site. There was no overt toxicity with levels of vanc 3600microgml and BMP-2 released, but the presence of vanc suppressed 20-50 BMP-2 induced alkaline phosphatase expression in cells. Additional work will need to determine retention of BMP by composite and long term effects of vancomycin on BMP-2 stimulated osteogenesis.
- Anatomy and Physiology
- Medicine and Medical Research