Accession Number:

ADA532546

Title:

A Novel Approach for Sub-Threshold Detection and Prevention of Laser Injury in Ocular Tissue

Descriptive Note:

Final rept. 15 Jan 2006-31 May 2009

Corporate Author:

TEXAS UNIV MEDICAL BRANCH AT GALVESTON

Report Date:

2009-05-31

Pagination or Media Count:

12.0

Abstract:

Aim I. Development of a novel fluorescence imaging technique, for in vivo detection of retinal pigment epithelium lesions following exposure to laser radiation. We acquired a new custom-built multi wavelength scanning laser ophthalmoscope SLO for multi spectral in vivo fluorescence imaging of animal retina following laser exposure. The imaging system was optimized for retinal imaging in aged Brown Norway rats. In order to induce laser lesions in the retina in vivo, we integrated the surgical laser system with our ophthalmoscope and we obtained images of the rat retina from animals following laser exposure. Aim II. Demonstrate that preconditioning of retina by using chronic low dose light exposure or transient sub threshold exposure to thermal insult induces a desirable adaptive response that could provide retina protection against subsequent supra-threshold laser exposure. Duplicate experiments suggested an effect of pre-conditioning, in that the heated animals required a higher laser power to show RPE changes by fundus autofluorescence. Subsequently, we found that post-exposure conditioning showed an even more consistent protection. Pre-and post conditioning upregulated Heat Shock Proteins effectively.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research
  • Lasers and Masers

Distribution Statement:

APPROVED FOR PUBLIC RELEASE