Site Specific Incorporation of Amino Acid Analogues into Proteins In Vivo
Final rept. 22 Jul 2004-11 Jul 2010
MASSACHUSETTS INST OF TECH CAMBRIDGE
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The goal of this project is to develop methods for the site-specific insertion in vivo of one or more amino acid analogues into proteins in eubacteria and in eukaryotes. The amino acids to be used include those that are photoactivatable, those that are fluorescent, those that carry reactive side chains such as keto groups, heavy atoms such as iodine, those that act as spectroscopic probes, and those that mimic phosphoserine, phosphothreonine, or phosphotyrosine. Besides providing a method for the production of new biomateirals with novel chemical and biological properties, proteins carrying such amino acid analogues will have wide applications in biology. These include studies on the folding, structure, stability and function of proteins, protein-protein interactions, protein localization and protein dynamics in vivo and signal transduction.
- Anatomy and Physiology