In Utero Influences, Breast Stem Cells, and Breast Cancer Risk Factors
Annual rept. 1 Aug 2008-31 Jul 2009
MASSACHUSETTS UNIV MEDICAL CENTER WORCESTER
Pagination or Media Count:
Breast density can be assessed by whole mount carmine alum staining of the fourth inguinal mammary glands according to the method reported by Brisken et al 2002 and counting the number of terminal end buds. Mammary glands of pregnant C57Bl6J mice were found to have significantly more terminal end buds than virgin mice. Flow cytometric analyses of dissociated cells from mammary glands clearly identified a sub-population of cells that are CD49f and CD24, putative markers of mammary stemprogenitor cells.
- Medicine and Medical Research