Accession Number:

ADA528307

Title:

Human Neural Cell-Based Biosensor

Descriptive Note:

Quarterly rept. 11 Jun-11 Sep 2010

Corporate Author:

ARUNA BIOMEDICAL INC ATHENS GA

Personal Author(s):

• Stice, Steven L.
• Chilton, Jamie
• Powe, Allan

Report Date:

2010-09-10

Pagination or Media Count:

5.0

Abstract:

Human neural progenitor cells have a strong potential for use as cell-based biosensors for environmental toxins. The overall goal of this project is to develop a human neural cell based biosensor using ArunAs neural progenitor cell line, hNP1-tm. In this report, we detail the following progress toward development of a human cell-based biosensor using these neural progenitor cells 1 we continue to develop methods for enriching neural progenitor populations using VVA lectin and antibodies against CNTFR-alpha, 2 we have begun to develop methods for directed differentiation of neural progenitors into motoneurons and astrocytes using defined medium conditions, and 3 we have begun development of fluorescence based assays for proliferation, mitochondrial function and reactive oxygen species generation.

Descriptors:

• *MOTOR NEURONS
• *NEUROTOXINS
• ANTIBODIES
• CELLS(BIOLOGY)
• TOXINS AND ANTITOXINS
• MITOCHONDRIA
• FLUORESCENCE
• ASTROCYTES
• AGGLUTININS
• NERVOUS SYSTEM
• OXYGEN GENERATORS

Subject Categories:

• Biochemistry
• Medicine and Medical Research
• Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE