Amplification of Type II Cadherins in Prostate Cancer
Final rept. 30 Oct 2005-29 Oct 2009
TEXAS UNIV HEALTH SCIENCE CENTER AT SAN ANTONIO
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Genomic alterations of 18q have been observed in prostate cancer. This research focused on analyzing the role of increased gene copy number at 18q22.1 found specifically in prostate cancer. We believe the key gene is the cellular adhesion gene cadherin 7 CDH7. Theincreased copies of CDH7 results in increased levels of CDH7 mRNA in tumor cells. We studiedthe role of loss of expression of CDH7 on the migratory and invasive potential of prostate cancer cells. We evaluated prostate cancer cells knocked-down for CDH7 expression in vitro and found that loss of CDH7 expression played no role in migratory or invasive potential. We created CDH7 monoclonal antibodies to study protein levels in prostate tumors. These antibodies were tested in immunohistochemistry IHC experiments with paraffin-embedded prostate tissue, but these antibodies were inadequate for IHC. Recently available commercial antibodies were also problematic. Due to the lack of adequate antibody reagents, our future direction will be an evaluation of the copy number of the CDH7 region in prostate tumors by deep sequencing to determine the exact target of the increased copy number at 18q22.1.
- Anatomy and Physiology
- Medicine and Medical Research