The AIF/XIAP Axis in Prostate Cancer
Annual rept. 15 Apr 2009-14 Apr 2010
WAKE FOREST UNIV WINSTON-SALEM NC SCHOOL OF HEALTH SCIENCES
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We have made substantial progress over the past year towards completion of the project goals both outlined in and extending beyond the original Statement of Work. We have made slow progress in establishing restoration cells expressing AIF variants with defined properties, in part due to difficulties associated with achieving successful lentiviral infection of target cells. In other studies, we have determined that AIF ablation has a significant impact on the ability of PC3 cells to grow and develop macroscopic clusters when plated in Matrigel. Further, the invasive characteristics of PC3 cells are substantially attenuated when AIF expression is suppressed. These data correlate well with studies presented in year 1, and suggest that not only is AIF necessary for the tumorigenic growth of prostate cancer cells in vivo, but also that the mechanism by which AIF affects tumor growth is through supporting the invasive growth of cells in 3D cultures. Our experiments to assess those properties of AIF that are most critical for prostate tumor growth continue to be underway these new data suggest a possible mechanism for AIF support of prostate cancer through augmenting the ability of prostate cancer cells to grow invasively.
- Medicine and Medical Research