Multidisciplinary Analysis of Cyclophilin A Function in Human Breast Cancer
Annual summary, 1 Mar 2009-28 Feb 2010
NORTHWESTERN UNIV EVANSTON IL
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The ongoing purpose of this work is to examine the effects of altered cyclophilin A CypA levels and activity on the prolactin receptor PRLr associated signaling and its role in breast cancer development andor progression. To this end, we have further demonstrated that siRNA-mediated CypA knockdown or the use of CypA inhibitors such as cyclosphorine A CsA analogs-CsD and CsH significantly abrogated the PRLr signaling and breast cancer cell growth in vitro. In addition, by using a novel antibody phage display technology, we have developed several synthetic antibodies, which exhibit nanomolar affinities for the CypA. Experiments are currently underway to test these potential synthetic antibodies in vitro and in vivo.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research