Role of Fetuin-A in Breast Tumor Cell Growth
Final rept. 31 Mar 2007-28 Feb 2010
MEHARRY MEDICAL COLL NASHVILLE TN
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In this final report we report that we successfully completed task 1, after doing additional experiments requested by the reviewers. The manuscript is presently under editorial review in the American Journal of Pathology. In the past year we have concentrated mainly on Task 2. The most significant reportable outcome of this task is that we have now removed doubts regarding the authenticity of fetuin-A as adhesion and growth signaling molecule. The purification protocol we have developed can purify fetuin-A to 100 purity. More importantly we have shown that human fetuin-A ahsg behaves exactly like its bovine counterpart. We have shown that in addition to mediating the activation of PI3 kinaseAkt, fetuin-A at high concentrations can also activate MAP kinases. We have also shown that in regular tissue culture, fetuin-A could be more relevant for cellular attachment than integrins. Fetuin-A is also actively internalized by the tumor cells but the significance of this internalization is presently unclear.
- Medicine and Medical Research