Accession Number:

ADA523882

Title:

Humanized in vivo Model for Autoimmune Diabetes

Descriptive Note:

Annual rept. 8 Jan 2009-7 Jan 2010

Corporate Author:

BENAROYA RESEARCH INST SEATTLE WA

Personal Author(s):

Report Date:

2010-02-01

Pagination or Media Count:

19.0

Abstract:

We previously reported now published, 2009 that two human GAD65 555-567-responsive T cell receptors TcRs expressed in HLA DR0401 transgenic mice exhibit different mechanisms of self-tolerance. 164 TcR transgenic mice evoke tolerance by strong thymic negative selection and peripheral activation-induced cell death AICD. 4.13 TcR transgenic mice select the TcR transgene near normal thymic cellularity levels and in the periphery result as a combination of IFN -secreting Th1 cells and IL-10-secreting Tr1 cells. Through crosses onto IL-10 knockout mice, we now have preliminary evidence that the regulatory cytokine IL-10 is maintaining an unactivated phenotype in 4.13 mice, which is in contrast to the self-antigenactivated phenotype observed in T cells from 164 mice.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE