Detection of Biological Warfare Agents in Municipal Tap Water via Standardized Culture Methods
Final rept. Jan 2004-Sep 2006
EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND MD RESEARCH AND TECHNOLOGY DIR
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Use of Laboratory Response Network LRN Level A culture methods was investigated for detection of biological warfare agents and their surrogates in municipal tap water. One hundred liters of water was concentrated 400 fold via ultrafiltration and spiked either before Phase I or after Phase II ultrafiltration with selected biological warfare agents. Aliquots of retentate were plated onto selective media, putative isolates were picked, and confirmed by standard microbiological and biochemical testing. For Phase I, limits of detection LODs were 10, 100-1,000, 500-750, and 750-1,000 CFUL for Bacillus anthracis, Burkholderia thailandensis, Yersinia pestis and Franciscella tularensis, respectively. For Phase II, LODs were 250, 500, 106 and 5,000 CFUL for B. anthracis, Burkholderia pseudomallei, Y. pestis, and F. tularensis, respectively. Overall, preconcentration followed by analyses using LRN Level A methods proved effective in not only detecting select agents or their simulants but also in determining the LOD in municipal water.
- Chemical, Biological and Radiological Warfare