Characterization of Physiological Roles and Prognostic Importance of IR/IGF-IR Hybrid Receptors in Breast Cancer
Annual summary rept. 15 Dec 2008-14 Dec 2009
BAYLOR COLL OF MEDICINE HOUSTON TX
Pagination or Media Count:
An inducible dimerization system will be used to study the IRIGF-IR Hybrid receptor by constructing chimeric IR and chimeric IGF-IR with different dimerization domains. MCF10A cells stably expressing chimeric IGF-IR were generated and homodimerizer AP20187 treatment was able to induce activation of chimeric IGF-IR and down stream AKT and ERK pathways. Retroviral vectors for chimeric IR with FKBP and FRB domains were constructed and will be used to created stable cell lines for further study. To measure the IRIGF-IR hybrid receptor on paraffin embedded specimens, a proximity ligation assay was used for dual detection of IRIGF-IR hybrid receptor using both anti-IR and anti-IGF-IR antibodies. The PLA assay was optimized in both cell pellet and breast tissue specimens to detect IRIGF-IR hybrid receptors and more studies will be done to confirm the specificity of the PLA assay.
- Medicine and Medical Research