Accession Number:

ADA522666

Title:

Human Neural Cell-Based Biosensor

Descriptive Note:

Quarterly technical rept. 12 Mar-11 Jun 2010

Corporate Author:

ARUNA BIOMEDICAL INC ATHENS GA

Report Date:

2010-06-11

Pagination or Media Count:

14.0

Abstract:

Human neural progenitors have a strong potential for use as cell-based biosensors for environmental toxins. In this report, we demonstrate that we have 1 optimized methods to genetically modify hNP1TM cells, 2 devised strategies for enriching the neural progenitor cell population using cell surface markers, and 3 developed methods for directed dopaminergic differentiation using defined medium conditions - all towards the goal of accelerating neuronal differentiation for biosensor development. Moreover, we have begun an exploration of fluorescence-based assays as a new direction for sensor element development, using neurite outgrowth as a test platform for screening neurotoxicants. Such exploration of alternate sensor elements beyond microelectrode arrays will broaden the utility of a human neural cell-based biosensor.

Subject Categories:

  • Medicine and Medical Research
  • Chemical, Biological and Radiological Warfare

Distribution Statement:

APPROVED FOR PUBLIC RELEASE