Accession Number:

ADA519642

Title:

High Volume Air Sampling for Viral Aerosols: A Comparative Approach

Descriptive Note:

Master's thesis

Corporate Author:

AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH GRADUATE SCHOOL OF ENGINEERING AND MANAGEMENT

Personal Author(s):

Report Date:

2010-03-01

Pagination or Media Count:

177.0

Abstract:

The United States Air Force has high volume biological air sampling equipment available including the XMX2L-MIL and DFU-1000. Neither system has been evaluated for effectiveness in the collection of viruses. Furthermore, decontamination methods have not been evaluated for these systems after use in sampling for a viral agent. MS2 bacteriophage was used as a surrogate virus. Aerosolized MS2 was released into a 12 m3 exposure chamber. High and moderate airborne concentrations of MS2 were evaluated. Low volume impingers were used for comparative purposes as well. Samples were analyzed using plaque assay and polymerase chain reaction PCR. At high viral loads the XMX2L-MIL and DFU-1000 achieved collection effectiveness equal to or greater than the low volume impingers. At moderate levels of airborne viral load, the XMX2L-MIL was capable of collecting viral quantities within 25 of the quantities collected by the low volume impingers. The DFU-1000 achieved marginal collection effectiveness of virus at moderate concentrations compared to the XMX2L-MIL and is considered to be unreliable in the quantification of viral agent at moderate levels and below. The DFU-1000 and XMX2L-MIL were capable of collecting detectable MS2 with PCR analysis at all concentrations. Ten percent sodium hypochlorite commercial bleach solution effectively decontaminated MS2.

Subject Categories:

  • Chemical, Biological and Radiological Warfare

Distribution Statement:

APPROVED FOR PUBLIC RELEASE