Accession Number:

ADA519474

Title:

Test for Chemical Induction of Chromosome Aberration in Cultured Chinese Hamster Ovary (CHO) Cells With and Without Metabolic Activation. Test Article: N,N,N',N'-tetramethyl Ethanediamine (TMEDA)

Descriptive Note:

Final

Corporate Author:

SITEK RESEARCH LABS ROCKVILLE MD

Personal Author(s):

Report Date:

2008-06-13

Pagination or Media Count:

73.0

Abstract:

N,N,N,N-tetramethyl ethanediamine TMEDA, 99.86 pure was tested for its potentials to induce chromosome aberrations in cultured Chinese Hamster Ovary CHO cells with and without metabolic activation according to OECD TG 473 in compliance with Good Laboratory Practice. TMEDA was tested at concentrations of 500, 1000, 2500 and 5000 microgramsmL in both with and without activation. It produced a positive response in this system with or without metabolic activation, but only at the highest concentration 5,000 microgramsmL However, according to the OECD guidelines TG 473, the compound is considered to be negative in the CHO chromosomal aberration assay, since the compound is not clastogenic at 0.01M 1,140 microgramsmL. A confirmatory chromosome aberration assay was performed without activation also showed negative at concentrations up to 3,000 microgramsmL but positive at the highest concentration.

Subject Categories:

  • Biology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE