Accession Number:

ADA519002

Title:

Single Assay for Simultaneous Detection and Differential Identification of Human and Avian Influenza Virus Types, Subtypes, and Emergent Variants

Descriptive Note:

Journal article

Corporate Author:

NAVAL HEALTH RESEARCH CENTER SAN DIEGO CA DEPT OF RESPIRATORY DISEASE RESEARCH

Report Date:

2010-02-01

Pagination or Media Count:

19.0

Abstract:

For more than four decades the cause of most type A influenza virus infections of humans has been attributed to only two viral subtypes, AH1N1 or AH3N2. In contrast, avian and other vertebrate species are a reservoir of type A influenza virus genome diversity, hosting strains representing at least 120 of 144 combinations of 16 viral hemagglutinin and 9 viral neuraminidase subtypes. Viral genome segment reassortments and mutations emerging within this reservoir may spawn new influenza virus strains as imminent epidemic or pandemic threats to human health and poultry production. Traditional methods to detect and differentiate influenza virus subtypes are either time-consuming and labor-intensive culture-based or remarkably insensitive antibody-based. Molecular diagnostic assays based upon reverse transcriptase-polymerase chain reaction RT-PCR have short assay cycle time, and high analytical sensitivity and specificity. However, none of these diagnostic tests determine viral gene nucleotide sequences to distinguish strains and variants of a detected pathogen from one specimen to the next. Decision-quality, strain- and variant-specific pathogen gene sequence information may be critical for public health, infection control, surveillance, epidemiology, or medicalveterinary treatment planning. The Resequencing Pathogen Microarray RPM-Flu is a robust, highly multiplexed and target gene sequencing-based alternative to both traditional culture- or biomarker-based diagnostic tests. RPM-Flu is a single, simultaneous differential diagnostic assay for all subtype combinations of type A influenza viruses and for 30 other viral and bacterial pathogens that may cause influenzalike illness. These other pathogen targets of RPM-Flu may co-infect and compound the morbidity andor mortality of patients with influenza.

Subject Categories:

  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE