Role of BRCA2 in the Expressions of IRF9-regulated Genes in Human Breast Cells
Annual rept. 1 Jul 2008-30 Jun 2009
MEHARRY MEDICAL COLL NASHVILLE TN
Pagination or Media Count:
We hypothesize that BRCA2 facilitates the formation andor the function of the ternary ISGF3 complex and thus, functional BRCA2 protein is essential for the antiproliferative effects of type I interferons against human breast tumor cells. Specific aims A To evaluate further the structural and functional interactions of BRCA2 with the members of the ISGF3 complex STAT1, STAT2 and IRF9 in the human breast cells. B To evaluate the antiproliferative effects of BRCA2 over expression in the human breast cells with or without knock down of the IRF9 protein by RNA interference. C To evaluate the antiproliferative effects of type I interferons against tumors developed by BRCA2 positive and BRCA2 negative human breast tumor cells in the nude mice xenograft model.
- Medicine and Medical Research