Recruitment & Regulation of N-Wasp by F-Bar Family Member CIP4 in Invasive Breast Cancer Cells
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TEXAS UNIV HEALTH SCIENCE CENTER AT HOUSTON
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The focus of this project is the contribution of the Cdc42-interacting protein CIP4 to the invasive phenotype of MDA-MB-231 cancer cells. CIP4 is a member of the F-BAR family of proteins, which interact with or induce plasma membrane curvature through their amphipathic BAR domain. My lab has previously shown that CIP4 interacts with N-WASp, a Cdc42 effector and actin polymerization promoter, which is implicated in the formation of, specialized invasive structures known as invadopodia. Invadopodia are actin-rich protrusions that combine cytoskeletal reorganization with localized degradation of extracellular matrix substrates to mediate cellular invasion. In my preliminary research, I noticed increased CIP4 expression in highly invasive breast cancer cell lines. My research investigates the role of CIP4 in promoting invasion and invadopodia in breast cancer cells in vitro through its interaction with N-WASp. The aims of the proposed work were 1 to determine the dynamics of CIP4 binding to N-WASp and their localization to the invadopodia, 2 to determine which domains of CIP4 are required for trafficking and activation of N-WASp, and 3 to examine the necessity of this interaction in the formation function of invadopodia and cellular invasion.
- Medicine and Medical Research