Regulation of the Inflammasome, a Modulator of Caspase-1 Mediated Cytokine Production
Annual summary rept. 1 Jul 2006-30 Jun 2007
PENNSYLVANIA UNIV PHILADELPHIA
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Tissue inflammation and inflammatory cytokines can positively affect breast cancer prognosis. By providing a detailed understanding of the mechanism of inflammasome formation and activation, we hope to create the potential for inflammation based cancer therapies. For each of the key protein domains in the inflammasome complex, panels of constructs have been screened for their amenity to further biochemical characterization. NALP LRR domain was found to express poorly, with poor solubility, possibly due to oxidation-related folding problems. Constructs of the adaptor CARDINAL were found to express well, but suffer proteolysis during purification. Analysis of CARDINAL partial proteolysis products may yield a proteolyticaly stable CARDINAL subdomain. NACHT constructs were successfully produced by baculovirusSF9 expression, and will be subjected to biochemical and enzymatic characterization.
- Medicine and Medical Research