Accession Number:

ADA517127

Title:

Regulation of the Inflammasome, a Modulator of Caspase-1 Mediated Cytokine Production

Descriptive Note:

Annual summary rept. 1 Jul 2006-30 Jun 2007

Corporate Author:

PENNSYLVANIA UNIV PHILADELPHIA

Personal Author(s):

Report Date:

2007-07-01

Pagination or Media Count:

7.0

Abstract:

Tissue inflammation and inflammatory cytokines can positively affect breast cancer prognosis. By providing a detailed understanding of the mechanism of inflammasome formation and activation, we hope to create the potential for inflammation based cancer therapies. For each of the key protein domains in the inflammasome complex, panels of constructs have been screened for their amenity to further biochemical characterization. NALP LRR domain was found to express poorly, with poor solubility, possibly due to oxidation-related folding problems. Constructs of the adaptor CARDINAL were found to express well, but suffer proteolysis during purification. Analysis of CARDINAL partial proteolysis products may yield a proteolyticaly stable CARDINAL subdomain. NACHT constructs were successfully produced by baculovirusSF9 expression, and will be subjected to biochemical and enzymatic characterization.

Subject Categories:

  • Medicine and Medical Research
  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE