Accession Number:

ADA513397

Title:

Plasma Membrane Permeabilization by Trains of Ultrashort Electric Pulses

Descriptive Note:

Corporate Author:

HUMAN PERFORMANCE WING (711TH)/RADIOFREQUENCY RADIATION (HPW/RHDR) BROOKS CITY-BASE TX

Report Date:

2009-01-01

Pagination or Media Count:

29.0

Abstract:

Ultrashort electric pulses USEP cause long-lasting increase of cell membrane electrical conductance, and that a single USEP increased cell membrane electrical conductance proportionally to the absorbed dose AD with a threshold of about 10 mJg. The present study extends quantification of the membrane permeabilization effect to multiple USEP and employed a more accurate protocol that identified USEP effect as the difference between post- and pre-exposure conductance values in individual cells. We showed that delta-g can be increased by either increasing the number of pulses at a constant E-field, or by increasing the E-field at a constant number of pulses. For 60-ns pulses, an E-field threshold of 6 kVcm for a single pulse was lowered to less than 1.7 kVcm by applying 100-pulse or longer trains. However, the reduction of the E-field threshold was only achieved at the expense of a higher AD compared to a single pulse exposure. Furthermore, the effect of multiple pulses was not fully determined by AD, suggesting that cells permeabilized by the first pulses in the train become less vulnerable to subsequent pulses. This explanation was corroborated by a model that treated multiple-pulse exposures as a series of single-pulse exposures and assumed an exponential decline of cell susceptibility to USEP as delta-g increased after each pulse during the course of the train.

Subject Categories:

  • Biology
  • Electricity and Magnetism
  • Plasma Physics and Magnetohydrodynamics

Distribution Statement:

APPROVED FOR PUBLIC RELEASE