Accession Number:

ADA513299

Title:

Pathogenesis of Aerosolized Eastern Equine Encephalitis Virus Infection in Guinea Pigs

Descriptive Note:

Journal article

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Report Date:

2009-01-01

Pagination or Media Count:

14.0

Abstract:

Mice and guinea pigs were exposed to aerosols containing either the NJ1959 or ArgM strains of Eastern Equine Encephalitis virus EEEV. Mice were more susceptible to either strain of aerosolized EEEV than were guinea pigs however, clinical signs indicative of encephalitis were more readily seen in guinea pigs. Lethality was lower for EEEV at large aerosol particle sizes 3 m although the differences in the median lethal dose LD50 were not statistically significant. Virus isolation and immunohistochemistry indicated that virus invaded the brains of guinea pigs within one day of aerosol exposure, regardless of virus strain or particle size. Immunohistochemistry further demonstrated that neuroinvasion occurred via the olfactory system, followed by transneuronal spread to all regions of the brain. Olfactory bipolar neurons and neurons throughout the brain were the key viral targets. The main microscopic lesions in infected guinea pigs were neuronal necrosis, inflammation of the meninges and neuropil of the brain and vasculitis in the brain. These results indicate that guinea pigs infected by aerosolized EEEV recapitulate several key features of fatal human infection and thus should serve as a suitable animal model for aerosol exposure to EEEV.

Subject Categories:

  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE