Role of Fetuin-A in Breast Tumor Cell Growth
Annual rept. 1 Mar 2008-29 Feb 2009
MEHARRY MEDICAL COLL NASHVILLE TN
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In this report, we have described the experiments pertaining to task1 of our original proposal To elucidate the significance of fetuin-A in breast carcinogenesis. The central hypothesis of this grant is that fetuin-A is a major serum derived growth factor for breast carcinoma cells and creates a favorable environment for the metastatic spread of tumor cells to the lungs. We have successfully placed FetPymT Fet-PymT and Fet--PymT female C57 black mice in experimental protocols. Our data show that lack of fetuin-A protects female fetuin-A null PymT mice from breast cancer. However, if fetuin-A null mice develop breast cancer, it is mainly squamous carcinoma, a rare breast cancer. We have also made significant progress in task 2 where we now show for the first time that cell attachment is mediated only by fetuin-A and not its major contaminant which is alpha-2-macroglobulin. We also show both fetuin-A and alpha-2-macroglobulin can activate PI3 kinaseAkt.
- Medicine and Medical Research