Mitochondrial Gene Cytochrome b Developmental and Environmental Expression in Aedes aegypti (Diptera: Culicidae)
AGRICULTURAL RESEARCH SERVICE GAINESVILLE FL CENTER FOR MEDICAL AGRICULTURAL AND VETERINARY ENTOMOLOGY
Pagination or Media Count:
Cytochrome b, coded by mitochondrial DNA, is one of the cytochromes involved in electron transport in the respiratory chain of mitochondria. Cytochrome b is a critical intermediate in a mitochondrial death pathway. To reveal whether cytochrome b of the mosquito Aedes aegypti L. Diptera Culicidae AeaCytB is developmentally regulated, we used real-time quantitative polymerase chain reaction qPCR to examine AeaCytB gene expression levels in different developmental stages of Ae. aegypti. The qPCR showed that AeaCytB was expressed in each developmental stage, with peaks at first and second instars and was highly expressed in teneral male and female Ae. aegypti adults. Because mitochondrial genes exist as multiple copies, AeaCytB has much higher expression levels in all developmental stages in Ae. aegypti compared with nuclear genes. We also investigated the effect of abiotic environmental factors e.g., high temperatures, ultraviolet radiation, and pesticide on AeaCytB gene expression. Taken together, these results suggest that AeaCytB gene plays an important role in the development of Ae. aegypti and its response to environmental stress.
- Genetic Engineering and Molecular Biology