Accession Number:

ADA512651

Title:

Naturally Occurring Peptide Zfra Inactivates Tumor Suppressors by Covalent Binding: An Act of Zfration

Descriptive Note:

Final rept. 1 Sep 2008-31 Aug 2009

Corporate Author:

NATIONAL CHENG KUNG UNIV TAINAN (TAIWAN)

Personal Author(s):

Report Date:

2009-09-01

Pagination or Media Count:

21.0

Abstract:

In this funded research, we have described zfration - a naturally occurring small-size peptide Zfra undergoes selfcovalent binding and aggregation, and may covalently interact with tumor suppressors for functional inactivation. Zfra is a 31-amino-acid peptide, whose composition is similar to that of zinc finger family proteins. In the original Specific Aims, we proposed to 1 determine how Zfra undergoes covalent self-aggregation and binding with tumor suppressor proteins for regulating their functions 2 determine the overall protein targets with which Zfra interact 3 investigate the effect of Zfra deficiency in breast cancer cells. As research ongoing as planned, we have achieved excellent progress and determined that Zfra can 1 induce degradation of intracellular zfrated proteins, 2 undergo enzyme-independent self-polymerization for more than 200 fold of its original size, and 3 significantly increase the growth of breast and skin basal cell carcinoma in nude mice by 3-4 fold in 3 months, suggestive of its inactivation of tumor suppressors in vivo.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research
  • Inorganic Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE