Identification of Species Related to Anopheles (Nyssorhynchus) albitarsis by Random Amplified Polymorphic DNA-Polymerase Chain Reaction (Diptera: Culicidae)
WALTER REED ARMY INST OF RESEARCH WASHINGTON DC DEPARTMENT OF ENTOMOLOGY
Pagination or Media Count:
Species-specific Random Amplified Polymorphic DNA-Polymerase Chain Reaction RAPD-PCR markers were used to identify four species related to Anopheles Nyssorhynchus albitarsis Lynch-Arribalzaga from 12 sites in Brazil and 4 in Venezuela. In a previous study Wilkerson et al. 1995, which included sites in Paraguay and Argentina, these four species were designated A, B, C and D. It was hypothesized that species A is An. Nys. albitarsis, species B is undescribed, species C is An. Nys. marajoara Galvao and Damasceno and species D is An. Nys. deaneorum Rosa-Freitas. Species D, previously characterized by RAPD-PCR from a small sample from northern Argentina and southern Brazil, is reported here from the type locality of An. Nys. deaneorum. Guajara-Mirim, State of Rondonia, Brazil. Species C and D were found by RAPD-PCR to be sympatric at Costa Marques, State of Rondonia, Brazil. Species A and C have yet to be encountered at the same locality. The RAPD markers for species C were found to be conserved over 4,620 km from Iguape, State of Sao Paulo, Brazil to Rio Socuavo, State of Zulia, Venezuela. RAPD-PCR was determined to be an effective means for the identification of unknown specimens within this species complex.