Frequency Domain Fluorimetry Using a Mercury Vapor Lamp
AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH SCHOOL OF ENGINEERING AND MANAGEMENT/DEPT OF ENGINEERING PHYSICS
Pagination or Media Count:
Frequency Domain FD fluorimetry, capitalizes on the frequency response function of a fluorophore and offers independence from light scatter and excitationemission intensity variations in order to extract the samples fluorescent lifetime. Mercury vapor lamps, a common source of industrial facility lighting, emit radiation that overlaps the UVblue absorption spectrum of many fluorophores and may be used as an efficient and portable excitation source. The AC power modulation of mercury vapor lamps modulates the lamps intensity at 120 Hz in the United States and higher harmonics. The fluorescent lifetimes for 3 different materials willemite, uranium doped glass and U3O8 are measured with conventional techniques and compared with the FD technique using the power harmonics from a mercury vapor lamp. The mercury lamp measurements agree to within 25 of the conventional methods.
- Metallurgy and Metallography
- Radiofrequency Wave Propagation