Accession Number:

ADA511852

Title:

Development of a Minimal-Bulk Oxygen Delivery Product to Enhance Survival During Hemorrhagic Shock/Studies Regarding the Use of Perfluorocarbon- Derived Intravascular Microbubbles from Oxygen Transport

Descriptive Note:

Final rept.

Corporate Author:

STATE UNIV OF NEW YORK AT BUFFALO AMHERST RESEARCH FOUNDATION

Personal Author(s):

Report Date:

2009-07-01

Pagination or Media Count:

49.0

Abstract:

Production of microbubble forming dodecafluoropentane emulsion DDFPe NuvOx Pharma LLC, Tucson, AZ was able to make high-quality emulsions February 2008, which have been successfully used in resuscitation of rats sever hemodilution and pigs potentially lethal hemorrhagic shock over the last 16 months. Goal 2 A shock model combining hemorrhage 32 mLkg total blood loss and a fractured femur bone was developed in pentobarbital anesthetized pigs weighing approximately 25 kg. In 19 pigs not given any treatment, 30 of the pigs survived for six hours. Goal 3 Comparison of Hextend plasma expander and DDFPe blood substitute for low volume resuscitation in the model developed during Goal 2. Eight out of ten pigs survived if Hextend was given 45 minutes after bleeding, suggesting a survival of 80. All six pigs given 0.6 mLkg DDFPe NuvOx Pharma LLC survived with good physiological values for more than six hours. This was interpreted as 100 of the DDFPe treated pigs survived for the required time. Repeatedly observed in Hextend treated pigs was development of severe pulmonary distress with foam in the lungs, dyspnea, and gradually rising content of CO2 and falling O2 tension in arterial blood. Goal 4 Semi-conscious pigs were bled 31.5 mLkg over 26 min and were allowed to develop shock over the next 35 min. Two randomly selected groups n6 each were resuscitated with either 7 mLkg Hextend or 0.6 mLkg DDFPe over 30 min. One pig died before any treatment was given. All treated pigs survived the experiments for 11 to 14 days, then euthanized. One of the Hextend treated pigs suffered severe sequel from the shock with no weight gain and was required euthanization on the 11th day. All DDFPe treated pigs were in good condition, increasing their weight at a normal rate.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE