Biological Sensors Using DNA Functionalized Multiwalled Carbon Nanotubes
Final rept. 25 Apr 2007-1 Oct 2009
INDIAN INST OF TECH MADRAS DEPT OF PHYSICS
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In the first part of this work, electrochemical detecting behavior of MWNT functionalized with single strand DNA was studied. The MWNT was synthesized as described in the report. Purified MWNT was functionalized with aqua regia for dispersion improve combining capacity with the DNA. Special machine grade DNA AC 15 was used for functionalization of MWNT by sonication. Characterizations have been carried out using various techniques. Cyclic voltammetry gave well-defined quasi-reversible voltametric responses, showing that DNA-MWNT film is a good electrochemical sensor. Further, we demonstrate highly selective and sensitive detection of a low concentration of dopamine. In the second part of this work, a new amperometric biosensor, based on deposition of glucose oxidase GOD on to crystalline gold Au nanoparticle modified multi walled carbon nanotube MWNT electrode, is presented. Purified MWNTs were decorated with nanocrystalline Au metal clusters using a simple chemical reduction method. Amperometric biosensor fabricated by depositing glucose oxidase GOD over Nafion-solubilized AuMWNT electrode retains its biocatalytic activity and offers fast and sensitive glucose quantification.
- Miscellaneous Detection and Detectors
- Atomic and Molecular Physics and Spectroscopy