Obstructing Androgen Receptor Activation in Prostate Cancer Cells through Posttranslational Modification by NEDD8
Annual rept. 15 Apr 2008-14 Apr 2009
ROBERT WOOD JOHNSON MEDICAL SCHOOL PISCATAWAY NJ
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In this study, we aim to investigate post-translational modification of the androgen receptor AR by a small ubiquitin-like protein, Nedd8. We planned to analyze the consequence of Nedd8 modification on AR activity, and to identify the neddylation sites on AR. We also planned to determine enzymes involved in AR neddylation and de-neddylation. We have completed all proposed tasks in the first year. Key accomplishments so far include the development of a protocol to enrich neddylated form of AR, the demonstration that a portion of endogenous AR is modified by Nedd8, and the finding that androgen reduces AR neddylation. Reportable outcomes include the preparation of a manuscript and the award of a PhD degree to a graduate student. We expect to continue the research into the second year and no major changes on future work are necessary.
- Medicine and Medical Research