CYP1B1 Polymorphism as a Risk Factor for Race-Related Prostate Cancer. Addendum
Final addendum rept. 1 Jun 2008-31 May 2009
NORTHERN CALIFORNIA INST FOR RESEARCH AND EDUCATION SAN FRANCISCO
Pagination or Media Count:
In the first hypothesis, CYP1B1 expression in Caucasian prostate cell lines were increased in cancerous DU145 PC-3 compared to normal RWPE-1 cells. Analysis of expression in human tissue cDNAs from Caucasians also showed higher levels of CYP1B1 in cancer compared to BPH. CYP1B1 protein was present in both races and though not significant, generally was higher in tumor regions compared to normal adjacent regions for both African-Americans and Whites. In the second hypothesis, racial differences for CYP1B1 polymorphisms are observed as allele frequencies for the variant at codons 119 and 432 are greater among Blacks P0.001 whereas the 453 variant is predominant in Whites P0.001. Within race, a case control study show the variant at codon 453 plays a protective role for PC among Blacks P0.05. Interestingly, SNPs at codons 432 and 449 are determined to be linked and the 432G-449C haplotype was observed to be a risk for PC P0.05. In a sampling of cases, no differences were observed between stages T2c vs T2c and grades 7 vs 7 of PC in either race. In the remaining year, more SNP studies with additional samples to be collected as well as further experimentation with aim 1 will be performed.
- Medicine and Medical Research