Age-Related DNA Methylation Changes and Neoplastic Transformation of the Human Prostate
Annual rept. 15 Jun 2008-14 June 2009
HOWARD UNIV WASHINGTON DC
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There is abundant evidence to suggest that DNA methylation changes may appear earlier during prostate cancer development than genetic changes, as well as more commonly and consistently. The purpose of the present study is to investigate whether aberrant methylation in normal prostate tissues may in itself be a pathologic event that increases with age. Using methylated CpG island amplification coupled with CpG promoter microarray, I have identified several novel genes that are differentially methylated in the human prostate. Quantitative methylation analysis using pyrosequencing technique shows hypermethylation of these genes in prostate cancer tissues compared with matched benign prostate tissues from the same patients. Furthermore, I observed methylation changes as a function of age for several genes and that the methylation profiles were different between samples from African American compared to Caucasian men. I have identified several novel genes as potential ethnic sensitive biomarkers for prostate cancer detection.
- Anatomy and Physiology
- Medicine and Medical Research