Ricin and Staphylococcal Enterotoxin B Fate in Water Matrices
Final rept. Oct 2004-Mar 2008
TEMPLE UNIV PHILADELPHIA PA SCHOOL OF MEDICINE
Pagination or Media Count:
This report is the final report for the Defense Threat Reduction Agency-funded project Biological Toxin Fate in Water Matrices BA05TAS080 and summarizes research directed at characterizing toxin stability in deionized water pH 7, 10 mM P04 with and without chlorine treatment in terms of hydrolysismass, denaturation, and activity as ascertained by polyacrylamide gel electrophoresis, circular dichroism, cell toxicity ricin, and cell activity SEB. Details of the research will be published separately. The SEB work was supplemented by liquid chromatography-mass spectrometry. Each toxin was stable for at least 30 days in 10 mM P04 pH 7 at room temperature. For each toxin, the majority of the toxin precipitated when heated slowly to the toxins Tm. The toxin remaining in solution retained some activity. Ricin remaining in solution maintained its native fold SEB remaining in solution did not. Each toxin retained some activity after treatment at low initial chlorine toxin molar ratios, with no activity observed for SEB after 5 day treatment with an initial chlorine toxin ratio of 501 and for ricin after 24 hr treatment with an initial chlorine toxin ratio of 1001. As the initial chlorine toxin ratio increased, the amount of native protein decreased.
- Physical Chemistry