MicroRNAs in Prostate Cancer
Final rept. 24 Oct 2005-23 Oct 2008
VIRGINIA UNIV CHARLOTTESVILLE
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A systematic comparison of the short RNA universe of two prostate cancer cell lines was performed by cloning and deep sequencing to identify microRNAs whose expression may correlate with androgen dependenceindependence or microRNAs that are regulated by androgens. The results were confirmed by microarrays and by quantitative reverse transcription and polymerase chain reaction. We have identified five microRNAs that are repressed and three that are induced during progression of prostate cancer from androgen dependent, early stage to androgen independent advanced stage. We have also identified four microRNAs that are repressed by androgens in the androgen dependent cells. The progression-repressed microRNAs render the advanced cancer cells unable to grow in charcoal-stripped androgen depleted serum. We have also identified several non-micro-short RNAs that are expressed abundantly in prostate cancer cells and provide evidence that one of them, tRF-1001, is necessary for cell proliferation.
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