Accession Number:

ADA505792

Title:

Chemical and Biological Sensing Utilizing Fused Bacteriorhodopsin Protein Hybrids

Descriptive Note:

Conference paper

Corporate Author:

MICHIGAN TECHNOLOGICAL UNIV HOUGHTON

Personal Author(s):

• Winder, Eric M.
• Griep, Mark H.
• Lueking, Donald R.
• Friedrich, Craig R.

Report Date:

2008-12-01

Pagination or Media Count:

7.0

Abstract:

This paper describes how monomeric bR can be overproduced in Escherichia coli and subsequently utilized as an integral component of a generic, nanoscale chemical sensing platform. The utility of this sensing platform is that it can be adapted for detection of a wide range of biological and chemical agents at, or below, nanomolar concentration levels. The gene encoding for bacteriorhodopsin has been successfully isolated from Halobacterium salinarum strain S9P using a colony-level PCR approach. Utilizing this purified DNA and a plasmid expression vector system, a fused protein hybrid consisting of maltose binding protein and bacterio-opsin has been expressed in transformed E. coli. The fusion hybrid has been purified in soluble form from E. coli cell-free extracts at up to 70mgL. Renaturation studies to incorporate all-trans retinal within the bacterio-opsin protein are currently underway.

Descriptors:

• *CHEMICAL DETECTION
• *BACTERIORHODOPSIN
• *PRODUCTION
• CHEMICAL AGENTS
• GENES
• PROTEINS
• BIOLOGICAL AGENTS
• SYMPOSIA
• ESCHERICHIA COLI

Subject Categories:

• Biochemistry
• Genetic Engineering and Molecular Biology
• Miscellaneous Detection and Detectors
• Chemical, Biological and Radiological Warfare

Distribution Statement:

APPROVED FOR PUBLIC RELEASE