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Nanosensors for Detection of Biological Threat Contaminants in Critical Buildings
CONSTRUCTION ENGINEERING RESEARCH LAB (ARMY) CHAMPAIGN IL
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A nanoscale sensing modality for detection of bacterial contaminants was developed. The modality uses fluorescent nanoparticles, also known as Quantum dots QDs conjugated to fragmented antibodies of targeted contaminants, and a quenching dye that labels an antigen surrogate. Coupling of the QD-labeled fragmented antibody and the quencher-labeled antigen allows sufficient fluorescent resonance energy transfer FRET to quench QD emissions. The subsequent addition of the targeted antigen displaces the dye-labeled bacteria, eliminating FRET and resulting in a concentration-dependent increase in QD photoluminescence. The novelty of this procedure lies in the applications for detection of a broad range of biological contaminants including viral and bacterial contaminants with a high degree of specificity and sensitivity. Furthermore, through the use of QDs of varying emission wavelengths the system can easily be adapted into a multiplexing immuno-assay.
APPROVED FOR PUBLIC RELEASE