Accession Number:

ADA505743

Title:

Nanosensors for Detection of Biological Threat Contaminants in Critical Buildings

Descriptive Note:

Conference paper

Corporate Author:

CONSTRUCTION ENGINEERING RESEARCH LAB (ARMY) CHAMPAIGN IL

Personal Author(s):

• Kumar, Ashok
• Stephenson, Larry D.
• Norton, Elizabeth
• Hale, Jeremy
• Nelson, Andrew

Report Date:

2008-12-01

Pagination or Media Count:

9.0

Abstract:

A nanoscale sensing modality for detection of bacterial contaminants was developed. The modality uses fluorescent nanoparticles, also known as Quantum dots QDs conjugated to fragmented antibodies of targeted contaminants, and a quenching dye that labels an antigen surrogate. Coupling of the QD-labeled fragmented antibody and the quencher-labeled antigen allows sufficient fluorescent resonance energy transfer FRET to quench QD emissions. The subsequent addition of the targeted antigen displaces the dye-labeled bacteria, eliminating FRET and resulting in a concentration-dependent increase in QD photoluminescence. The novelty of this procedure lies in the applications for detection of a broad range of biological contaminants including viral and bacterial contaminants with a high degree of specificity and sensitivity. Furthermore, through the use of QDs of varying emission wavelengths the system can easily be adapted into a multiplexing immuno-assay.

Descriptors:

• *QUANTUM DOTS
• *BIOLOGICAL AGENT DETECTORS
• *BIOLOGICAL CONTAMINATION
• *PHOTOLUMINESCENCE
• *NANOSTRUCTURES
• THREATS
• FRAGMENTATION
• BACTERIA
• BUILDINGS
• ENERGY TRANSFER
• TARGETS
• QUENCHING
• RESONANCE
• BIOLOGICAL WARFARE
• CONTAMINANTS
• ANTIBODIES
• DYES
• VIRUSES
• FLUORESCENCE
• DETECTION
• SYMPOSIA
• REPRINTS
• FREQUENCY
• EMISSION

Subject Categories:

• Organic Chemistry
• Electrical and Electronic Equipment
• Chemical, Biological and Radiological Warfare

Distribution Statement:

APPROVED FOR PUBLIC RELEASE