Accession Number:

ADA505713

Title:

Early Systemic Biomarkers of Acute Lung Injury: Application of Multiplex Proteomic Array Technology

Descriptive Note:

Conference paper

Corporate Author:

WALTER REED ARMY INST OF RESEARCH SILVER SPRING MD

Personal Author(s):

• Nath, Jayasree
• Januszkiewicz, Adolph

Report Date:

2008-12-01

Pagination or Media Count:

8.0

Abstract:

The primary objectives of the present study were 1 to set up a non-invasive animal model of inhalation injury, to monitor early changes in levels of pro-inflammatory cytokines in breath condensates of live rats, to delineate early biomarkers of pulmonary inflammation 2 to further investigate the role of specific proinflammatory mediators in acute lung inflammationinjury following exposure to toxic fire gases andor BOP shockwave-mediated blunt trauma 3 to monitor changes in various pro-inflammatory cytokineschemokines in small volumes of biological samples 10-50 microliters, by utilizing a highly sensitive Proteome Array-based Multiplex ELISA 4 to identify early systemic biomarkers of acute lung injurytoxicity, for development of much-needed strategiestools for effective therapeutic intervention and lung-health assessment of active-duty U.S. solders deployed around the world. The primary goal of this study was to identify early biomarkers of acute pulmonary inflammation, as an predictive indicator of acute lung toxicity andor injury, following exposure of rats to a brief high-dose of NO2, a militarily relevant toxic fire gas and a product of high temperature combustion.

Descriptors:

• *LUNG
• *INFLAMMATION
• *TOXIC AGENTS
• GASES
• NITROGEN DIOXIDE
• PROTEOMICS
• WOUNDS AND INJURIES
• SYMPOSIA
• INHALATION
• TOXICITY
• MODELS
• CYTOKINES

Subject Categories:

• Biochemistry
• Toxicology
• Weapons Effects (Biological)

Distribution Statement:

APPROVED FOR PUBLIC RELEASE