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Molecular Phylogeny of Neotropical Anopheles (Nyssorhynchus) albitarsis Species Complex (Diptera: Culicidae)
WALTER REED ARMY INST OF RESEARCH SILVER SPRING MD DEPT OF ENTOMOLOGY
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A phylogeny was reconstructed for four species belonging to the Neotropical Anopheles Nyssorhynchus albitarsis complex using partial sequences from the mitochondrial cytochrome oxidase I COI and NADH dehydrogenase 4 ND4 genes and the ribosomal DNA ITS2 and D2 expansion region of the 28S subunit. The basis for initial characterization of each member of the complex was by correlated random amplification of polymorphic DNA polymerase chain reaction HAPD-PCR markers. Analyses were carried out with and without an outgroup An. Nys. argyritarsis Robineau Desvoidy by using maximum parsimony, maximum likelihood. and Bayesian methods. A total evidence approach without the outgroup, using separate models for fast COI and ND4 position 3 and slow rDNA ITS2 and D2, and COl and ND4 position 1 partitions, gave the best supported topology, showing close relationships of An. albitarsis Lynch-Arribalzaga to An. albitarsis B and An. marajoara Galvao Damasceno to An. deaneorum Rosa-Freitas. Analyses with the outgroup included showed poorer support, possibly because of a long branch attraction effect caused by a divergent outgroup, which caused one of the All. marajoara specimens to cluster with An. deaneorum in some analyses. The relationship of the above-mentioned result to a separately proposed hypothesis suggesting a fifth species in the complex is discussed.
APPROVED FOR PUBLIC RELEASE