Accession Number:

ADA505409

Title:

Molecular Confirmation of the Specific Status of Anopheles halophylus (Diptera: Culicidae) and Evidence of a New Cryptic Species within An. triannulatus in Central Brazil

Descriptive Note:

Journal article

Corporate Author:

WALTER REED ARMY INST OF RESEARCH SILVER SPRING MD DEPT OF ENTOMOLOGY

Personal Author(s):

• Silva-do-Nascimento, T. F.
• Wilkerson, R. C.
• Lourenco-de-Oliveira, R.
• Monteiro, F. A.

Report Date:

2006-01-01

Pagination or Media Count:

6.0

Abstract:

Anopheles halophylus Silva-do-Nascimento Lourenc o-de-Oliveira was recently described using morphological and biological variants in specimens previously identi ed as Anopheles triannulatus Neiva Pinto. Because these two species occur in sympatry in central Brazil, we used allozymes to determine the extent of gene flow to confirm that they are different species. Of 11 allozyme loci analyzed, one Mpi was found to be diagnostic for An. halophylus and An. triannulatus, confirming their speci c status. This locus revealed a second sibling species within An. triannulatus sensu lato. An. halophylus and the new undescribed species were con rmed using random ampli ed polymorphic DNA markers that showed moderate genetic divergence among these three sympatric and closely related taxa D 0.145 0.428. Moreover, this marker indicates that An. halophylus and the new species are more closely related to each other than either is to An. triannulatus.

Descriptors:

• *GENES
• *CULICIDAE
• *ANOPHELES
• *DIPTERA
• *ENDANGERED SPECIES
• TAXONOMY
• POLYMORPHISM
• BRAZIL
• DISEASE VECTORS
• LOCUS
• MALARIA
• MARKERS
• DEOXYRIBONUCLEIC ACIDS
• MORPHOLOGY
• REPRINTS
• BIOLOGY

Subject Categories:

• Biology
• Medicine and Medical Research
• Ecology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE