Accession Number:

ADA505079

Title:

Nanothermite-Based Microsystem for Drug Delivery and Cell Transfection

Descriptive Note:

Conference paper

Corporate Author:

MISSOURI UNIV-COLUMBIA DEPT OF ELECTRICAL ENGINEERING

Personal Author(s):

• Apperson, S.
• Thiruvengadathan, R.
• Bezmelnitsyn, A.
• Gangopadhyay, K.
• Gangopadhyay, S.
• Polo-Parada, L.

Report Date:

2008-12-01

Pagination or Media Count:

9.0

Abstract:

This paper describes a new system for pressure-induced cell transfection. The system generates pressure pulses from a microchip that contains a small quantity of nanothermite material and an electrical igniter. The pressure output from the nanothermite reaction is coupled to the biological target through a PDMS membrane and a tube filled with gelatin. The system generates pressure pulses in the range of 10-40MPa. It has been used to transfect primary cells with 99 transfection rate and cell survival. It has also been used to transfect cell lines Hela, HL-60, and HT-29. In all cases survival was 99, and transfection rate in Hela, HL60 and HT-29 was up to 37 60 and 30 respectively. In addition, the system has been shown to transfect intact spinal cords and arteries from chicken St 30 with no noticeable damage.

Descriptors:

• *IGNITERS
• *CELLS(BIOLOGY)
• *TRANSFECTION
• *CHIPS(ELECTRONICS)
• ELECTRICAL PROPERTIES
• SURVIVAL(GENERAL)
• PULSES
• PRESSURE
• DRUGS
• CHICKENS
• ARTERIES
• SPINAL CORD
• GELATINS
• PRIMARY BATTERIES
• TARGETS
• RATES
• SURVIVABILITY
• OUTPUT
• DELIVERY

Subject Categories:

• Medicine and Medical Research
• Medical Facilities, Equipment and Supplies
• Electrical and Electronic Equipment

Distribution Statement:

APPROVED FOR PUBLIC RELEASE