DID YOU KNOW? DTIC has over 3.5 million final reports on DoD funded research, development, test, and evaluation activities available to our registered users. Click HERE
to register or log in.
Nanothermite-Based Microsystem for Drug Delivery and Cell Transfection
MISSOURI UNIV-COLUMBIA DEPT OF ELECTRICAL ENGINEERING
Pagination or Media Count:
This paper describes a new system for pressure-induced cell transfection. The system generates pressure pulses from a microchip that contains a small quantity of nanothermite material and an electrical igniter. The pressure output from the nanothermite reaction is coupled to the biological target through a PDMS membrane and a tube filled with gelatin. The system generates pressure pulses in the range of 10-40MPa. It has been used to transfect primary cells with 99 transfection rate and cell survival. It has also been used to transfect cell lines Hela, HL-60, and HT-29. In all cases survival was 99, and transfection rate in Hela, HL60 and HT-29 was up to 37 60 and 30 respectively. In addition, the system has been shown to transfect intact spinal cords and arteries from chicken St 30 with no noticeable damage.
APPROVED FOR PUBLIC RELEASE