The AIF/XIAP Axis in Prostate Cancer
Annual rept. 15 Apr 2008-14 Apr 2009
WAKE FOREST COLL WINSTON-SALEM NC
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In the past year significant progress has been made towards completion of the project goals as outlined in the original statement of work. We have succeeded in establishing PC3-derived cell lines that are deficient in protein expression for apoptosis inducing factor AIF, X-linked inhibitor of apoptosis XIAP, and both proteins simultaneously. AIF-deficient cells have been further characterized, and we have determined that these cells display no differences in either sensitivity to death- inducing stimuli or in vitro growth characteristics when compared to control cells. When implanted subcutaneously into immunocompromised mice, AIF-deficient PC3 cells display significant impairment in both the establishment rate and tumor growth characteristics in vivo. These data suggest that while AIF protein expression is not required for cell growth in culture, AIF plays a significant role in supporting the ability of PC3 cells to establish tumors and grow in vivo. Overall these data support the hypothesis that AIF supports prostate cancer pathogenesis, and experiments currently underway will determine those properties of AIF that are most critical for prostate tumor growth.
- Anatomy and Physiology
- Medicine and Medical Research