The Role of ABC Proteins in Drug Resistant Breast Cancer Cells
Annual summary rept. 14 Mar 2008-13 Mar 2009
GEORGETOWN UNIV WASHINGTON DC
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This predoctoral training grant, supported by the Department of Defense Breast Cancer Research Program BCRP of the Office of the Congressionally Directed Medical Research Programs CDMRP, aims to study the possible role of membrane transporters in pleitropic drug resistance, using a combination of molecular biological, biochemical, and biophysical methods. The third year of funding was spent in further characterization of the Plasmodium falciparum Chloroquine Transporter PfCRT, a member of a drugmetabolite transporter superfamily. Data from the previous year provided proof of the proteins direct interaction with its drug substrate. I have subsequently employed a methodology commonly applied to drug resistance proteins such as human P-glycoprotein and defined the drug binding site through mass spectrometry. This report summarizes work conducted from March 2008 through June 2008, when my work on this project ceased due to graduation. A request for a change of PI for this award has been submitted and is pending review.
- Medicine and Medical Research