A Quantitative in Vitro Assay for Chemical Mosquito-Deterrent Activity Without Human Blood Cells
ARMY MEDICAL DEPT BOARD FORT SAM HOUSTON TX
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We report that an aqueous solution containing 1023 M adenosine triphosphate ATP and citrate-phosphate-dextrose-adenine CPDA-1 can effectively replace transfusable human red blood cells in an in vitro Klun and Debboun bioassay system for evaluating chemicals for mosquito feeding-deterrent activity, using either Aedes aegypti or Anopheles stephensi. These species fed with similar avidity through collagen membrane covering aqueous 1023 M ATP plus CPDA-1 or red blood cells in CPDA-1 supplemented with ATP. In a 2nd experiment, we evaluated the feeding-deterrent activity of N,N-diethyl- 3-methylbenzamide and a newly discovered natural product chemical, --isolongifolenone, against these 2 mosquito species. We found that the feeding-deterrent efficacy of the 2 chemicals was similar whether the feeding stimulant was red blood cells supplemented with ATP or ATP alone with CPDA-1. Since the use of human red blood cells in bioassays raises important health and logistic issues, aqueous ATP with CPDA-1 is a reasonable alternative to human blood cells for routine in vitro chemical screening.
- Medicine and Medical Research