Intragenomic rDNA ITS2 Variation in the Neotropical Anopheles (Nyssorhynchus) albitarsis Complex (Diptera: Culicidae)
WALTER REED ARMY INST OF RESEARCH SILVER SPRING MD DEPT OF ENTOMOLOGY
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We cloned and sequenced the rDNA internal transcribed spacer 2 ITS2 of 4 species belonging to the neotropical Anopheles Nyssorhtnchus albitarsiscomplex, that is, A. albitarsis, A. albitarsis B Anopheles marajoara, a proven malaria vector and Anopheles deaneorum, a suspected vector. Even though the ITS2 sequences of these species were very similar - 1.17 divergence, we found differences suitable for species identification and intragenomic variation of possible consequence in phylogenetic reconstruction. Variation came from 2 microsatellite regions and a number of indels and base substitutions. The existence of partially correlated subsets of clones in A. albitarsis is hypothesized either to be separate rDNA loci or to be semi-independently evolving portions of a single rDNA locus. No differences were found between males and females, suggesting that similar rDNA arrays exist on both the X and Y chromosomes. In addition, highly variant clones, possibly. pseudogenes, were found in A. marajoara from Venezuela.
- Medicine and Medical Research