A Population Genetics Study of Anopheles Darlingi (Diptera: Culicidae) from Colombia Based on Random Amplified Polymorphic DNA-Polymerase Chain Reaction and Amplified Fragment Length Polymorphism Markers
WALTER REED ARMY INST OF RESEARCH SILVER SPRING MD DEPT OF ENTOMOLOGY
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The genetic variation and population structure of three populations of Anopheles darlingi from Colombia were studied using random amplified polymorphic markers RAPDs and amplified fragment length polymorphism markers AFLPs. Six RAPD primers produced 46 polymorphic fragments, while two AFLP primer combinations produced 197 polymorphic fragments from 71 DNA samples. Both of the evaluated generic markers showed the presence of gene flow, suggesting that Colombian An. darlingi populations are in panmixia. Average genetic diversity, estimated from observed heterozygosity, was 0.374 RAPD and 0.309 AFLP. RAPD and AFLP markers showed little evidence of geographic separation between eastern and western populations however, the FsubST valued showed high gene flow between the two western populations RAPD FsubST 0.029 Nm 8.5 AFLP FsubST 0.051 Nm 4.7. According to molecular variance analysis AMOVA, the genetic distance between populations was significant RAPD PhisubST 0.084 AFLP PhisubST 0.229, P 0.001. The FsubST distances and AMOVAs using AFLP loci support the differentiation of the Guyana biogeographic province population from those of the Choco-Magdalena. In this last region, Choco and Cordoba populations showed the highest genetic flow.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research