Genomic and Expression Profiling of Benign and Malignant Nerve Sheath Tumors in Neurofibromatosis Patients
Final rept. 1 May 2003-31 Apr 2008
STANFORD UNIV CA
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The goal of the study is to identify genes that will serve as molecular markers for progression of neurofibroma to MPNST, and to identify potential therapeutic targets. miRNA expression profiling was performed on 6 cases of MPNSTs, and 7 cases of synovial sarcomas. By using unsupervised hierarchical clustering most tumors were grouped together according to tumor type. Subsequent analyses using Significance Analysis of Microarrays SAM identified miRNAs that differentiate between MPNSTs and synovial sarcoma SS. To develop a cell line model for MPNSTs, global gene expression profiles for cell lines established from 3 primary MPNST and SS tumor tissues was carried out and their expression profiles were compared with other sarcomas. A large tissue microarray TMA containing about 200 nerve sheath tumors was used to test for EGFR expression by IHC. Neoplasms in which the majority of samples showed high expression by IHC included MPNST 83 of NF1- associated and 77 of sporadic, 73 of plexiform neurofibroma, 100 diffuse neurofibroma and 93 of SS.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research