Assessment of GPR30, a Seven Transmembrane-spanning Estrogen Receptor, as an Oncogene
Annual rept. 18 Sep 2006-17 Sep 2007
RHODE ISLAND HOSPITAL PROVIDENCE
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Our prior work has linked the seven transmembrane estrogen receptor GPR30 to specific estrogen binding Thomas et al, 2005, rapid estrogen action Filardo and Thomas, 2005 and the development of metastatic breast cancer in man Filardo et al, 2006. To further address the role of GPR30 in experimental breast cancer biology, transgenic mice were created for the purpose of overexpressing wild type or active GPR30 in the mammary gland using the mouse mammary tumor virus tissue specific promoter. Two suitable candidate founder mice with stably integrated wild-type GPR30 were generated T6- 1A and T6-2E. We were unsuccessful in our attempts to generate an active GPR30 allele as assessed by in vitro assays outline in this proposal during this time frame. A no cost extension was requested to further propagate the wild-type mice and to analyze their capacity to develop mammary adenocarcinoma.
- Medicine and Medical Research